Primary Antibody Labeling Kit

  • FAST-DNA-PAINT Kit
    Primary Antibody Labeling Kit

    MASSIVE-sdAB-FAST 1-PLEX

    This kit provides ready-to-use binders for FAST DNA-PAINT imaging (FAST-PAINT) of mouse IgG kappa light chain or rabbit IgG primary antibodies. Also included are imager strands and buffers required for immunostaining and DNA-PAINT imaging.

    This kit provides one camelid single-domain antibody (sdAB) coupled to a highly optimized DNA-PAINT site called a FAST sequence. FAST sequences decrease image acquisition time by at least 3-fold and provide a higher signal-to-noise ratio than standard sequences. Each sdAB is site-specifically coupled to a single FAST sequence and two sdABs can bind one primary antibody, resulting in two docking sites per primary antibody.

    Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.

    Protocol/Datasheet for MASSIVE-sdAB-FAST 1-PLEX

    400

  • FAST-DNA-PAINT Kit
    Primary Antibody Labeling Kit

    MASSIVE-sdAB-FAST 2-PLEX

    This kit provides ready-to-use binders for FAST-DNA-PAINT imaging (FAST-PAINT) of mouse IgG kappa light chain and rabbit IgG primary antibodies. Also included are imager strands and buffers required for immunostaining and DNA-PAINT imaging.
    This kit provides two camelid single-domain antibodies (sdABs) for DNA-PAINT coupled to highly optimized DNA-PAINT sites called FAST sequences. FAST sequences decrease image acquisition time by at least 3-fold and provide a higher signal-to-noise ratio than standard sequences. Each sdAb is site-specifically coupled to a single FAST docking site and two sdABs can bind one primary antibody, resulting in two docking sites per primary antibody.

    Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.

    Protocol/Datasheet for MASSIVE-sdAB-FAST 2-PLEX

    700

  • DNA-PAINT Kit
    Primary Antibody Labeling Kit

    MASSIVE-sdAB 1-PLEX

    This kit contains one camelid single-domain antibody (sdABs) for DNA-PAINT that targets either mouse IgG kappa light chain or rabbit IgG. Also included are imager strands and buffers that are required for sample preparation and imaging. 

    The sdAb is site-specifically coupled to a single DNA-PAINT docking site and two sdAbs can bind one primary antibody, resulting in two docking site per primary antibody. The small size of secondary camelid sdABs leads to a reduced linkage-error and increased accessibility of primary antibody labeled proteins. 

    Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.

    Protocol/Datasheet for MASSIVE-sdAB 1-PLEX.

    400

  • DNA-PAINT Kit
    Primary Antibody Labeling Kit

    MASSIVE-sdAB 2-PLEX

    This kit contains two camelid single-domain antibodies (sdABs) for DNA-PAINT that target mouse IgG kappa light chain and rabbit IgG. Also included are imager strands and buffers that are required for sample preparation and imaging. 

    Each sdAB is site-specifically coupled to a single DNA-PAINT docking site and two sdABs can bind one primary antibody, resulting in two docking site per primary antibody. The small size of secondary camelid sdAbs leads to a reduced linkage-error and increased accessibility of primary antibody labeled proteins. 

    Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.

    Protocol/Datasheet for MASSIVE-sdAB 2-PLEX

    700

    • Microtubules stained
    DNA-PAINT Kit
    Primary Antibody Labeling Kit

    MASSIVE-AB 1-PLEX

    This kit contains polyclonal secondary antibodies coupled to DNA-PAINT docking sites that target mouse, rabbit or rat IgG. Also included are imager strands and buffers that are required for sample preparation and imaging.

    As each antibody is conjugated to multiple (3-4) DNA strands and several antibodies can bind a single primary antibody, these reagents provide strong signal amplification of your protein target.

    Secondary antibodies binding to other species than mouse or rabbit are available upon request.  

    Protocol/Datasheet for MASSIVE-AB 1-PLEX.

    400

    • HSP60 and TOM 20 stained.
    • Microtubules and clathrin stained with MASSIVE-AB 2-PLEX by Christophe Leterrier/Karoline Friedl, NeuroCyto, INP, Marseille
    DNA-PAINT Kit
    Primary Antibody Labeling Kit

    MASSIVE-AB 2-PLEX

    This kit contains polyclonal secondary antibodies coupled to DNA-PAINT docking sites that target mouse, rabbit or rat IgG. Also included are imager strands and buffers that are required for sample preparation and imaging.

    As each antibody is conjugated to multiple (3-4) DNA strands and several antibodies can bind a single primary antibody, these reagents provide strong signal amplification of your protein target.

    Secondary antibodies binding to other species than mouse or rabbit are available upon request.

    Protocol/Datasheet for MASSIVE-AB 2-PLEX.

    700

  • Microtubles. TOM20 and PMP70 stained with MASSIVE-AB 2-PLEX kit
    DNA-PAINT Kit
    Primary Antibody Labeling Kit

    MASSIVE-AB 3-PLEX

    This kit provides ready-to-use antibody binders for multiplexed DNA-PAINT imaging of 3 cellular targets via exchangePAINT. Also included are imager strands and buffers required for sample preparation and imaging.

    This kit contains polyclonal secondary antibodies that target mouse, rabbit, and rat IgG coupled to standard DNA-PAINT sites/sequences. Each antibody is conjugated to multiple (3-4) DNA strands and several antibodies can bind a single primary antibody, providing strong signal amplification of your target.

    Secondary antibodies that bind other species are available upon request under “Custom Conjugation”.

    Protocol/Datasheet for MASSIVE-AB 3-PLEX.

    850

  • DNA-PAINT Kit
    Primary Antibody Labeling Kit

    DNA-PAINT starter kit

    DNA-PAINT is a localization based super-resolution imaging technique with several advantages.
    It offers:

    • Sub-20-nm spatial resolution
    • No photobleaching
    • No photoswitching of dyes necessary
    • Compatible with standard microscopes
    • Easy spectral multiplexing

    Step into the world of DNA-PAINT with our starter-kit containing one ready-to-use GATTA-PAINT 80(R or Y) nanoruler slide and one MASSIVE-AB 1-PLEX labeling kit for 1-color  (ATTO 655 or Cy3B) DNA-PAINT measurements in biological cells. Please find further information below.

    Collaboration product between GATTAquant and Massive Photonics.

    500

  • Primary Antibody Labeling Kit
    ONI DNA-PAINT kit

    MASSIVE-SDAB ONI 2-PLEX

    This DNA-PAINT kit has been optimized for the ONI Nanoimager microscope. The kit provides ready-to-use binders for DNA-PAINT imaging of mouse IgG kappa light chain and rabbit IgG primary antibodies. Also included are imager strands and buffers required for immunostaining and DNA-PAINT imaging.
    This kit includes two camelid single-domain antibodies (sdABs) for DNA-PAINT, each coupled to unique FAST sequences optimized for the ONI Nanoimager. FAST sequences decrease image acquisition time by at least 3-fold and provide a higher signal-to-noise ratio than our standard sequences. Each sdAB is site-specifically coupled to a single FAST docking site and two sdABs can bind one primary antibody, resulting in two docking sites per primary antibody.

    Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.

    Protocol/Datasheet for MASSIVE-sdAB ONI 2-PLEX

    700