MASSIVE-sdAB-FAST 1-PLEX

This kit provides ready-to-use binders for FAST DNA-PAINT imaging (FAST-PAINT) of mouse IgG kappa light chain or rabbit IgG primary antibodies. Also included are imager strands and buffers required for immunostaining and DNA-PAINT imaging.

This kit provides one camelid single-domain antibody (sdAB) coupled to a highly optimized DNA-PAINT site called a FAST sequence. FAST sequences decrease image acquisition time by at least 3-fold and provide a higher signal-to-noise ratio than standard sequences. Each sdAB is site-specifically coupled to a single FAST sequence and two sdABs can bind one primary antibody, resulting in two docking sites per primary antibody.

Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.

Protocol/Datasheet for MASSIVE-sdAB-FAST 1-PLEX