TECHNOLOGY

WHAT IS DNA-PAINT?

DNA-PAINT (DNA-Point Accumulation for Imaging in Nanoscale Topography) is a localization based super-resolution microscopy technique. By the transient binding of a fluorophore modified DNA strand (imager) to a target labeled with complementary DNA strand (docking site) blinking of the fluorophores can be observed on the camera of a fluorescence microscope. By recording the blinking for a couple of minutes and exactly fitting the localization in each frame a super-resolved image of your specimen can be created.

Microtubules stained

To label cells secondary antibodies (AB) (or single-domain antibodies (sdAB)) with specific docking sites are required. To support your research and make DNA-PAINT wider available in the field for super-resolution cell imaging Massive Photonics is providing ready-to-use kits for DNA-PAINT cell microscopy.
A Massive Photonics kit consist out of a specific secondary antibody with a DNA-PAINT docking site (AB), fluorophore labeled imager and all necessary buffers (washing, imaging, and antibody incubation buffer).

WORKFLOW

The secondary AB binds to your primary antibody labeled cell and makes it available for DNA-PAINT imaging. After incubation with the imager (usually in the lower nM range) this starts to diffuse to the docking site. The binding and unbinding can be observed. Only when the imager binds to the docking site of your AB the fluorophore can be detected on the camera chip of your microscope which called ON time.

WHY DNA-PAINT?

  • Sub-20-nm spatial resolution
  • No photobleaching
  • No photoswitching of dyes necessary
  • Compatible with standard microscopes
  • Easy spectral multiplexing