This FAST-DNA-PAINT kit enables super-resolution imaging of target proteins tagged with the HaloTag® *. This kit includes ready-to-use single-domain antibody (sdAB) binders against HaloTag® and required buffers and imagers for immunostaining and DNA-PAINT imaging. The anti-Halo sdAB is site-specifically coupled to one FAST sequence (F4) and recognizes a surface-exposed epitope on the HaloTag®, making it compatible with simultaneous Halo ligand binding. This kit is optimized for imaging at 21-25 °C. Please inquire if imaging at higher temperatures.
FAST sequences are optimized to decrease image acquisition time and increase signal-to-noise ratios. Choose between two FAST imager strand options: FAST or FAST-LB. FAST sequences enable ~15 nm resolution using 50-75 ms exposure times. FAST-LB imagers enable ~10 nm resolution using 100-150 ms exposure times.
sdABs developed and manufactured by NanoTag Biotechnologies are sold under license. Unmodified and fluorophore- coupled sdABs (FluoTag®) remain available for direct purchase from NanoTag Biotechnologies.
Protocol/Datasheet for MASSIVE-TAG-Q-FAST anti-Halo
* HaloTag® is a registered trademark of Promega Corporation.
400€
This FAST-DNA-PAINT kit enables super-resolution imaging of RFP-tagged proteins. This kit includes ready-to-use single-domain antibody (sdAB) binders against RFP and required buffers for immunostaining and DNA-PAINT imaging. Each anti-RFP sdAB is site-specifically coupled to one FAST sequence (F3) and one sdAB binds per RFP protein. This kit is optimized for imaging at 21-25 °C. Please inquire if imaging at higher temperatures.
FAST sequences are optimized to decrease image acquisition time and increase signal-to-noise ratios. Choose between two FAST imager strand options: FAST or FAST-LB. FAST sequences enable ~15 nm resolution using 50-75 ms exposure times. FAST-LB imagers enable ~10 nm resolution using 100-150 ms exposure times.
sdABs developed and manufactured by NanoTag Biotechnologies are sold under license. Unmodified and fluorophore- coupled sdABs (FluoTag®) remain available for direct purchase from NanoTag Biotechnologies.
400€
This kit provides ready-to-use binders for FAST DNA-PAINT imaging (FAST-PAINT) of target proteins tagged with GFP and its most common derivatives. This kit also contains imager strands, and buffers required for immunostaining and DNA-PAINT imaging of GFP-tagged proteins
This kit contains single-domain antibody (sdAB) binders against GFP that are coupled to highly optimized DNA-PAINT sites called FAST sequences. FAST sequences decrease image acquisition time by at least 3-fold and provide a higher signal-to-noise ratio than standard sequences. Each sdAB is coupled to a single DNA-PAINT FAST sequence and and one sdAB can bind one GFP.
Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.
400€
This kit provides ready-to-use binders for FAST DNA-PAINT imaging (FAST-PAINT) of target proteins tagged with GFP and its most common derivatives. This kit also contains imager strands, and buffers required for immunostaining and DNA-PAINT imaging of GFP-tagged proteins.
This kit contains a mixture of two different clones of sdABs that target GFP and its most common derivatives. Up to two sdABs can bind one GFP, and each sdAB is coupled to a highly optimized DNA-PAINT site called a FAST sequence. FAST sequences decrease image acquisition time by at least 3-fold and provide a higher signal-to-noise ratio than standard sequences.
Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.
400€
This kit contains a single-domain antibody (sdAB) binding to GFP and its most common derivatives. Each antibody is coupled to a single DNA-PAINT docking site thus very suitable for quantitative (Q) measurements. This kit also contains imager strands, and buffers required for immunostaining and DNA-PAINT imaging of GFP-tagged proteins.
Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.
400€
This kit provides ready-to-use single-domain antibody (sdAB) binders for DNA-PAINT imaging that target GFP-tagged proteins.
This kit contains a mixture of two different clones of sdABs that bind GFP and it’s most common derivatives. Up to two sdABs can bind one GFP, and each sdAB is coupled to one DNA-PAINT docking site, providing signal amplification of your target. This kit also contains imager strands, and buffers required for immunostaining and DNA-PAINT imaging of GFP-tagged proteins.
Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.
400€
This kit provides ready-to-use binders for DNA-PAINT imaging of target proteins tagged with the 12 amino acid ALFA tag (Nature Commun. 2019). Also included are imager strands and buffers that are required for sample preparation and imaging.
This kit contains single-domain antibody (sdAB) binders that recognize ALFA tag and are coupled to standard DNA-PAINT sites/sequences.
Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.
400€
This kit provides ready-to-use binders for FAST DNA-PAINT (FAST-PAINT) imaging of target proteins tagged with the 12 amino acid ALFA tag (Nature Commun. 2019). Also included are FAST imager strands and buffers that are required for sample preparation and imaging.
This kit contains single-domain antibody (sdAB) binders that recognize ALFA tag and are coupled to FAST-PAINT sequences.
Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.
400€
This kit provides ready-to-use binders for DNA-PAINT imaging of target proteins tagged with the fluorescent protein TagFP and its common derivatives. Also included are imager strands and buffers required for sample preparation and imaging.
This kit contains single-domain antibody (sdAB) binders that recognize TagFP and are coupled to standard DNA-PAINT sites/sequences. Each sdAB is coupled to a single DNA-PAINT site.
Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.
400€
This kit provides ready-to-use binders for DNA-PAINT imaging of target proteins tagged with the fluorescent protein mEOS and its common derivatives. Also included are imager strands and buffers required for sample preparation and imaging.
This kit contains single-domain antibody (sdAB) binders coupled to standard DNA-PAINT sites/sequences. Each sdAB is coupled to a single DNA-PAINT site.
Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.
400€
This kit provides ready-to-use single-domain antibody (sdAB) binders for RESI (Resolution Enhancement by Sequential Imaging) of ALFA-tagged proteins.
RESI is a DNA-PAINT based method that achieves sub-nanometer spatial-resolution in cells, enabling precise imaging of individual proteins even in high-density environments.
The ALFA-tag is a small rationally designed protein tag that facilitates highly specific labeling.
Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.
From 850€
This kit provides ready-to-use single-domain antibody (sdAB) binders for RESI (Resolution Enhancement by Sequential Imaging) of GFP-tagged proteins. RESI enables users the highest possible resolutions (<1 nm).
This kit contains anti-GFP sdABs conjugated to 4 different RESI docking sites. One sdAB can bind one GFP, and each sdAB is coupled to one DNA-PAINT docking site.
Anti-GFP sdABs bind GFP and its most common derivatives. This kit also comes with imager strands, and buffers required for RESI of GFP-tagged proteins.
Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.
From 850€
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