FAST-DNA-PAINT Kit

  • FAST-DNA-PAINT Kit
    Primary Antibody Labeling Kit

    MASSIVE-sdAB-FAST 1-PLEX

    This kit provides ready-to-use binders for FAST DNA-PAINT imaging (FAST-PAINT) of mouse IgG kappa light chain or rabbit IgG primary antibodies. Also included are imager strands and buffers required for immunostaining and DNA-PAINT imaging.

    This kit provides one camelid single-domain antibody (sdAB) coupled to a highly optimized DNA-PAINT site called a FAST sequence. FAST sequences decrease image acquisition time by at least 3-fold and provide a higher signal-to-noise ratio than standard sequences. Each sdAB is site-specifically coupled to a single FAST sequence and two sdABs can bind one primary antibody, resulting in two docking sites per primary antibody.

    Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.

    Protocol/Datasheet for MASSIVE-sdAB-FAST 1-PLEX

    400

  • FAST-DNA-PAINT Kit
    Primary Antibody Labeling Kit

    MASSIVE-sdAB-FAST 2-PLEX

    This kit provides ready-to-use binders for FAST-DNA-PAINT imaging (FAST-PAINT) of mouse IgG kappa light chain and rabbit IgG primary antibodies. Also included are imager strands and buffers required for immunostaining and DNA-PAINT imaging.
    This kit provides two camelid single-domain antibodies (sdABs) for DNA-PAINT coupled to highly optimized DNA-PAINT sites called FAST sequences. FAST sequences decrease image acquisition time by at least 3-fold and provide a higher signal-to-noise ratio than standard sequences. Each sdAb is site-specifically coupled to a single FAST docking site and two sdABs can bind one primary antibody, resulting in two docking sites per primary antibody.

    Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.

    Protocol/Datasheet for MASSIVE-sdAB-FAST 2-PLEX

    700

  • FAST-DNA-PAINT Kit
    Fluorescent Protein and Tag Labeling Kit

    MASSIVE-TAG-Q-FAST anti-GFP

    This kit provides ready-to-use binders for FAST DNA-PAINT imaging (FAST-PAINT) of target proteins tagged with GFP and its most common derivatives. This kit also contains imager strands, and buffers required for immunostaining and DNA-PAINT imaging of GFP-tagged proteins
    This kit contains single-domain antibody (sdAB) binders against GFP that are coupled to highly optimized DNA-PAINT sites called FAST sequences. FAST sequences decrease image acquisition time by at least 3-fold and provide a higher signal-to-noise ratio than standard sequences. Each sdAB is coupled to a single DNA-PAINT FAST sequence and and one sdAB can bind one GFP.

    Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.

    Protocol/Datasheet for MASSIVE-TAG-Q-FAST anti-GFP.

    400

  • FAST-DNA-PAINT Kit
    Fluorescent Protein and Tag Labeling Kit

    MASSIVE-TAG-X2-FAST anti-GFP

    This kit provides ready-to-use binders for FAST DNA-PAINT imaging (FAST-PAINT) of target proteins tagged with GFP and its most common derivatives. This kit also contains imager strands, and buffers required for immunostaining and DNA-PAINT imaging of GFP-tagged proteins.

    This kit contains a mixture of two different clones of sdABs that target GFP and its most common derivatives. Up to two sdABs can bind one GFP, and each sdAB is coupled to a highly optimized DNA-PAINT site called a FAST sequence. FAST sequences decrease image acquisition time by at least 3-fold and provide a higher signal-to-noise ratio than standard sequences.

    Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.

    Protocol/Datasheet for MASSIVE-TAG-X2-FAST anti-GFP.

    400

  • FAST-DNA-PAINT Kit
    Fluorescent Protein and Tag Labeling Kit

    MASSIVE-TAG-Q-FAST anti-ALFA

    This kit provides ready-to-use binders for FAST DNA-PAINT (FAST-PAINT) imaging of target proteins tagged with the 12 amino acid ALFA tag (Nature Commun. 2019). Also included are FAST imager strands and buffers that are required for sample preparation and imaging.

    This kit contains single-domain antibody (sdAB) binders that recognize ALFA tag and are coupled to FAST-PAINT sequences.

    Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.

    Protocol/Datasheet for MASSIVE-TAG-Q-FAST anti-ALFA.

    400

  • FAST-DNA-PAINT Kit
    Direct Target Labeling Kit

    Massive-sdAB-FAST anti-PDL1

    This kit provides ready-to-use binders for DNA-PAINT imaging of PD-L1, also known as Programmed Cell Death Ligand 1. PD-L1 is a transmembrane protein that plays a critical role in immune regulation by binding to its receptor PD-1 on T cells. Also included are imager strands and buffers for sample preparation and imaging.

    This kit contains single-domain antibody (sdAB) binders that recognize PD-L1 and are coupled to FAST DNA-PAINT sequence F1. Each sdAB is coupled to a single DNA-PAINT docking strand.

    sdABs developed and manufactured by NanoTag Biotechnologies are sold under license. Unmodified and fluorophore- coupled sdABs (FluoTag®) remain available for direct purchase from NanoTag Biotechnologies.

    Protocol/Datasheet for MASSIVE-sdAB-FAST anti-PDL1

    400

  • FAST-DNA-PAINT Kit
    Direct Target Labeling Kit

    Massive-sdAB-FAST anti-CTLA4

    This kit provides ready-to-use binders for DNA-PAINT imaging of CTLA-4, also known as CD152 or Cytotoxic T-Lymphocyte Associated Antigen 4, an immune checkpoint receptor that regulates T cell activation. CTLA-4 inhibits immune responses by binding to co-stimulatory molecules. Also included are imager strands and buffers for sample preparation and imaging.

    This kit contains single-domain antibody (sdAB) binders that recognize CTLA4 and are coupled to FAST DNA-PAINT sequence F2. Each sdAB is coupled to a single DNA-PAINT docking strand.

    sdABs developed and manufactured by NanoTag Biotechnologies are sold under license. Unmodified and fluorophore- coupled sdABs (FluoTag®) remain available for direct purchase from NanoTag Biotechnologies.

    Protocol/Datasheet for MASSIVE-sdAB-FAST anti-CTLA4

    400

  • FAST-DNA-PAINT Kit
    Direct Target Labeling Kit

    Massive-sdAB-FAST anti-CD45

    This kit provides ready-to-use binders for DNA-PAINT imaging of CD45, also known as Leukocyte Common Antigen (LCA). CD45 is a receptor-linked protein tyrosine phosphatase expressed on leukocytes, where it is essential for regulating immune cell signaling. Also included are imager strands and buffers for sample preparation and imaging.

    This kit contains single-domain antibody (sdAB) binders that recognize CD45 and are coupled to FAST DNA-PAINT sequence F3. Each sdAB is coupled to a single DNA-PAINT docking strand.

    sdABs developed and manufactured by NanoTag Biotechnologies are sold under license. Unmodified and fluorophore- coupled sdABs (FluoTag®) remain available for direct purchase from NanoTag Biotechnologies.

    Protocol/Datasheet for MASSIVE-sdAB-FAST anti-CD45

    400

    FAST-DNA-PAINT Kit
    Direct Target Labeling Kit
    Neuro-Imaging Kit

    MASSIVE-sdAB-FAST anti-Synaptotagmin 1

    This kit provides ready-to-use binders for DNA-PAINT imaging of Synaptotagmin-1, a transmembrane protein enriched at pre-synaptic vesicles. Also included are imager strands and buffers for sample preparation and imaging.

    This kit contains single-domain antibody (sdAB) binders that recognize Synaptotagmin-1 and are coupled to the FAST-DNA-PAINT sequence F1. Each sdAB is coupled to a single DNA-PAINT docking site.

    Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.

    Protocol/Datasheet for MASSIVE-sdAB-FAST anti-Synaptotagmin 1

    400

  • FAST-DNA-PAINT Kit
    Direct Target Labeling Kit
    Neuro-Imaging Kit

    Massive-sdAB-FAST anti-VGLUT1

    This kit provides ready-to-use binders for DNA-PAINT imaging of the vesicular glutamate transporter 1 (VGLUT1), which localizes to the membrane of synaptic vesicles of excitatory glutamatergic synapses. Also included are imager strands and buffers for sample preparation and imaging.

    This kit contains single-domain antibody (sdAB) binders that recognize VGLUT1 and are coupled to FAST DNA-PAINT sequence F2. Each sdAB is coupled to a single DNA-PAINT docking strand.

    Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.

    Protocol/Datasheet for MASSIVE-sdAB-FAST anti-VGLUT1

    400

    FAST-DNA-PAINT Kit
    Direct Target Labeling Kit
    Neuro-Imaging Kit

    Massive-sdAB-FAST anti-PSD95

    This kit provides ready-to-use binders for DNA-PAINT imaging of the major scaffolding Postsynaptic Density Protein 95 or PSD95 (alternative names: PSD-95, SAP-90 and DLG4), which localizes at post-synaptic densities of excitatory synapses. Also included are imager strands and buffers for sample preparation and imaging.

    This kit contains single-domain antibody (sdAB) binders that recognize PSD-95 and are coupled to FAST-DNA-PAINT sequence F3. Each sdAB is coupled to a single DNA-PAINT docking strand.

    Unmodified and fluorophore coupled sdABs (FluoTag®) available at NanoTag.

    Protocol/Datasheet for MASSIVE-sdAB-FAST anti-PSD95

    400

  • FAST-DNA-PAINT Kit
    Direct Target Labeling Kit
    Neuro-Imaging Kit

    Massive-AB-FAST anti-Bassoon

    This kit provides ready-to-use binders for DNA-PAINT imaging of the scaffolding protein Bassoon, which is essential for the organization of the presynaptic active zone and the regulation of neurotransmitter release. Also included are imager strands and buffers for sample preparation and imaging.

    This kit contains antibodies recognizing Bassoon that are preincubated with single-domain antibodies (sdABs) conjugated to FAST-DNA-PAINT sequence F4.

    Unmodified ABs available at Synaptic Systems.

    Protocol/Datasheet for MASSIVE-AB-FAST anti-Bassoon

    550

  • FAST-DNA-PAINT Kit
    Direct Target Labeling Kit
    Neuro-Imaging Kit

    Massive-AB-FAST anti-Tau

    This kit provides ready-to-use binders for DNA-PAINT imaging of the neuron-specific microtubule-associated protein Tau, which stabilizes microtubules in axons and acts as a distinctive axonal marker. Also included are imager strands and buffers for sample preparation and imaging.

    This kit contains antibodies recognizing Tau that are preincubated with single-domain antibodies (sdABs) conjugated to FAST-DNA-PAINT sequence F5.

    Unmodified ABs available at Synaptic Systems.

    Protocol/Datasheet for MASSIVE-AB-FAST anti-Tau

    550

  • FAST-DNA-PAINT Kit
    Direct Target Labeling Kit
    Neuro-Imaging Kit

    Massive-AB-FAST anti-Map2

    This kit provides ready-to-use binders for DNA-PAINT imaging of the neuron-specific Microtubule-associated protein 2 (Map2), which plays a key role in stabilizing microtubules in dendrites and acts as a specific dendritic marker. Also included are imager strands and buffers for sample preparation and imaging.

    This kit contains antibodies recognizing Map2 that are preincubated with single-domain antibodies (sdABs) conjugated to FAST-DNA-PAINT sequence F6

    Unmodified ABs available at Synaptic Systems.

    Protocol/Datasheet for MASSIVE-AB-FAST anti-Map2

    550