Massive-sdAB-FAST Anti-Human

This FAST-DNA-PAINT kit enables super-resolution imaging of Human IgGs. This kit includes ready-to-use single-domain antibody (sdAB) binders against Human IgGs and required buffers and imagers for immunostaining and DNA-PAINT imaging. The anti-Human sdAB is site-specifically coupled to one FAST sequence (F4) and recognizes the Fc domain of Human IgG1, IgG3, and IgG4 isotypes with high specificity and affinity. This kit is optimized for imaging at 21-25 °C. Please inquire if imaging at higher temperatures.

FAST sequences are optimized to decrease image acquisition time and increase signal-to-noise ratios. Choose between two FAST imager strand options: FAST or FAST-LB. FAST sequences enable ~15 nm resolution using 50-75 ms exposure times. FAST-LB imagers enable ~10 nm resolution using 100-150 ms exposure times.

sdABs developed and manufactured by NanoTag Biotechnologies are sold under license. Unmodified and fluorophore- coupled sdABs (FluoTag®) remain available for direct purchase from NanoTag Biotechnologies.

Protocol/Datasheet for MASSIVE-sdAB anti-Human