Labeling kits contain standard or FAST sequences. All sequences within individual categories are orthogonal. Standard sequences are orthogonal to FAST imagers. FAST imagers with the same number in their name aren’t orthogonal, ex: F1, F1-LB, F1-FG).

Our kit portfolio includes have 6 different FAST sequences and 5 different standard sequences. However, we offer more DNA-PAINT sequences are available upon request.

Yes, we provide custom imagers with a wide range of dyes. Simply send the imager dye and sequence to info@massive-photonics.com for pricing and a lead time estimate.

Yes, please inquire at info@massive-photonics.com.

All Standard and FAST-LB (Long Binding) sequences are compatible with MINFLUX DNA-PAINT imaging. Standard sequences in “DNA-PAINT kits”. FAST-LB sequences in “FAST kits”.

Our kits contain antibodies (ABs), single-domain antibodies (sdABs or nanobodies), or small proteins conjugated to DNA-PAINT sequences. Detailed information found in product datasheets.

ABs are approximately 150 kDa and 15 nm × 10 nm (L × H), and are conjugated to multiple (3-4) DNA strands. Several secondary ABs can bind a single primary AB to provide strong signal amplification of your protein target.

sdABs, also called nanobodies, are approximately 15 kDa and 2 nm × 3 nm (L × H). They minimize linkage error and enhance target accessibility compared to ABs. sdABs are conjugated to one DNA strand, and up to two secondary sdABs can bind one primary AB. sdABs against protein tags (ex: GFP, RFP, ALFA-tag) bind their target once (TAG-Q products) or twice (TAG-X2 products).

We use proprietary sequences and are unable to disclose the exact DNA sequences. Information about binding kinetics or sequence orthogonality with non-Massive Photonics sequences available upon request. We suggest ordering custom products if you must know exact sequences.

TAG-Q and TAG-X2 denote differences in the number of single-domain antibody (sdAB), i.e. nanobody, clones in the product. TAG-Q products contain one clone of the sdAB, which bind the target once. TAG-X2 product contain two clones of the sdAB, meaning up to two sdABs can bind each target.

Yes, please inquire at info@massive-photonics.com.

A method called pre-mixing (also called: pre-incubation) (https://pubs.rsc.org/en/content/articlehtml/2020/nr/d0nr00227e) enables multiple primary antibodies of the same species to be used in one sample. We have validated pre-incubation on our secondary anti-rabbit IgG and anti-mouse kLC single-domain antibodies. Please see the Website category “Multiplexing” for 1 – 6 Plex imaging kits and a free sample prep protocol.

To use our products for imaging at higher temperatures, you need a special imager sequence. Please let us know your imaging temperature and we’ll suggest the best imager

When publishing, simply add the name of the product and the DNA-PAINT sequence used. Example: Massive-sdAB 2-PLEX (Massive Photonics) was used to label primary antibodies X and X, and DNA-PAINT imaging was performed with imagers X-dye and X-dye, respectively.