DNA-PAINT (DNA-Point Accumulation for Imaging in Nanoscale Topography) is a localization based super-resolution microscopy method that uses short, dye-labeled single stranded DNA (ssDNA strands) that transiently bind to their complements:
- Imager strand – a dye-labeled DNA oligo present in imaging buffer
- Docking strand – a DNA oligo complementary to the imager strand that is delivered to the target of interest via binders (e.g. antibodies, single domain antibodies)