Massive-TAG-X2 Bruker anti-GFP

This FAST-DNA-PAINT kit has been specifically optimized for DNA-PAINT imaging on the Bruker Vutara VXL super-resolution microscope, which delivers exceptional 3D sub-diffraction imaging performance via bi-plane detection. This kit provides ready-to-use binders for FAST DNA-PAINT imaging of target proteins tagged with GFP and its most common derivatives. Also included are imager strands and buffers required for immunostaining and DNA-PAINT imaging of GFP-tagged proteins.

This kit provides a mixture of two different clones of single-domain antibody (sdAB) binders against GFP, each site-specifically conjugated to a unique FAST docking sequence and supplied with a fluorogenic Cy3B imager optimized for the Vutara VXL platform. Fluorogenic imagers reduce background fluorescence, improving signal-to-noise ratio — well suited to the bi-plane detection approach of the Vutara VXL. Up to two sdABs can bind one GFP, each coupled to a single FAST docking sequence, resulting in up to two docking sites per target protein.

sdABs developed and manufactured by NanoTag Biotechnologies are sold under license. Unmodified and fluorophore- coupled sdABs (FluoTag®) remain available for direct purchase from NanoTag Biotechnologies.

Protocol/Datasheet for MASSIVE-TAG-X2 Bruker anti-GFP